Cancer Letters
Volume 125, Issue 1 , Pages 31-34, 13 March 1998

Report of a hitherto unreported sequence present and expressed in a cancer cell line

Department of Biophysics, Molecular Biology and Genetics, University of Calcutta, 92, APC Road, Calcutta 700009, India

Received 22 February 1997; received in revised form 5 November 1997; accepted 5 November 1997.

Abstract 

During the amplification of a 202 base pair (bp) fragment around the 61 st codon of the N-ras gene, an extra band of about 150 bp in length was observed when genomic DNA from a human epidermoid carcinoma cell line was used as template. This fragment was cloned and sequenced. However, this sequence was not found in the databank. RT-PCR experiments indicated that the sequence is expressed in the cells about 8 h after serum induction. The relevant RNA hybridized to one strand of the sequence but not to the other.

Keywords:  Polymerase chain reaction, Hep2 cell line, N-ras, Novel sequence, Expression

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PII: S0304-3835(97)00501-6

Cancer Letters
Volume 125, Issue 1 , Pages 31-34, 13 March 1998

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