MicroRNA-10b induced by Epstein-Barr virus-encoded latent membrane protein-1 promotes the metastasis of human nasopharyngeal carcinoma cells

Li, Gang; Wu, Zhengrong; Peng, Ying; Liu, Xiong; Lu, Juan; Wang, Lu; Pan, Qiuhui; He, Min-Liang; Li, Xiang-Ping

doi:10.1016/j.canlet.2010.07.021

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Volume 299, Issue 1 , Pages 29-36, 18 December 2010

MicroRNA-10b induced by Epstein-Barr virus-encoded latent membrane protein-1 promotes the metastasis of human nasopharyngeal carcinoma cells

Gang Li
- Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
- Gang Li and Zhengrong Wu made equal contributions to this study.
Zhengrong Wu
- Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China
- Gang Li and Zhengrong Wu made equal contributions to this study.
Ying Peng
- Department of Neurology, The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China
Xiong Liu
- Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
Juan Lu
- Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
Lu Wang
- Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
Qiuhui Pan
- Medical Research Center, The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China
Min-Liang He
- Stanley Ho Center for Emerging Infectious Diseases, School of Public Health and Primary Care, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong, China
Xiang-Ping Li
- Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
- Corresponding author. Tel.: +86 20 61642001.

Received 22 May 2010; received in revised form 21 July 2010; accepted 23 July 2010. published online 23 August 2010.

Abstract

MicroRNA-10b (miR-10b) has been reported to facilitate the metastasis of breast cancer. However, little is known about the role of miR-10b in the metastasis of nasopharyngeal carcinoma (NPC). Here, we show that high levels of miR-10b expression in Epstein-Barr virus (EBV)-positive latent membrane protein-1 (LMP1)-expressing NPC cells, and its expression is down-regulated by silencing LMP1 or Twist. Induction of miR-10b over-expression in LMP1-silent C666-1 cells promoted significant wound healing and transmembrane invasiveness in vitro. More importantly, miR-10b over-expression promoted the metastasis of NPC and accelerated the death of tumor-bearing nude mice. These findings strongly suggest that miR-10b positively regulates the metastasis of NPC.

Keywords: miR-10b, Latent membrane protein-1, Twist, Nasopharyngeal carcinoma, Metastasis