Application of bioluminescence imaging to therapeutic intervention of herpes simplex virus type I – Thymidine kinase/ganciclovir in glioma

Jang, Su Jin; Kang, Joo Hyun; Kim, Kwang Il; Lee, Tae Sup; Lee, Yong Jin; Lee, Kyo Chul; Woo, Kwang Sun; Chung, Wee Sup; Kwon, Hee Chung; Ryu, Chun Jeih; Choi, Tae Hyun; Choi, Chang Woon; Lim, Sang Moo; Cheon, Gi Jeong

doi:10.1016/j.canlet.2010.04.028

« Previous Next »Cancer Letters
Volume 297, Issue 1 , Pages 84-90, 1 November 2010

Application of bioluminescence imaging to therapeutic intervention of herpes simplex virus type I – Thymidine kinase/ganciclovir in glioma

Su Jin Jang
- Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul 139-706, Republic of Korea
- Department of Biotechnology, Sejong University, Seoul 143-747, Republic of Korea
Joo Hyun Kang
- Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul 139-706, Republic of Korea
Kwang Il Kim
- Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul 139-706, Republic of Korea
Tae Sup Lee
- Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul 139-706, Republic of Korea
Yong Jin Lee
- Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul 139-706, Republic of Korea
Kyo Chul Lee
- Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul 139-706, Republic of Korea
Kwang Sun Woo
- Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul 139-706, Republic of Korea
Wee Sup Chung
- Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul 139-706, Republic of Korea
Hee Chung Kwon
- Laboratory of Molecular Oncology, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul 139-706, Republic of Korea
Chun Jeih Ryu
- Department of Biotechnology, Sejong University, Seoul 143-747, Republic of Korea
Tae Hyun Choi
- Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul 139-706, Republic of Korea
Chang Woon Choi
- Department of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul 139-706, Republic of Korea
Sang Moo Lim
- Department of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul 139-706, Republic of Korea
Gi Jeong Cheon
- Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul 139-706, Republic of Korea
- Department of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul 139-706, Republic of Korea
- Corresponding author at: Molecular Imaging Research Center and Department of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences (KIRAMS), 75 Nowon-gil, Gongneung-Dong, Nowon-Gu, Seoul 139-706, Republic of Korea. Tel.: +82 2 970 1273; fax: +82 2 970 1341.

Received 17 February 2010; received in revised form 21 April 2010; accepted 30 April 2010. published online 07 June 2010.

Abstract

Lentiviral vector containing the HSV1-tk and firefly luciferase (fLuc) gene was infected into C6 and C6-TL expressing HSV1-tk and fLuc gene was generated. C6-TL showed higher [¹²⁵I]IVDU uptake than C6. The survival rate of C6-TL decreased more rapidly with increasing GCV dose and was well correlated with fLuc activity. The images of microPET clearly demonstrated higher uptake of [¹⁸F]FHBG into the C6-TL tumor. Inhibition of tumor growth was observed in C6-TL tumor-bearing mice treated with GCV through tumor size measurement and bioluminescence imaging. The therapeutic effect of HSV1-tk/GCV system can be monitored using bioluminescent imaging and tumor size measurement.

Keywords: Glioma, HSV1-tk, Luciferase, Molecular imaging, Suicidal gene therapy