Tuning of apoptosis-mediator gene transcription in HepG2 human hepatoma cells through an adenosine signal

Yang, Dongqin; Yaguchi, Takahiro; Lim, Chun-Ren; Ishizawa, Youhei; Nakano, Takashi; Nishizaki, Tomoyuki

doi:10.1016/j.canlet.2009.10.016

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Volume 291, Issue 2 , Pages 225-229, 28 May 2010

Tuning of apoptosis-mediator gene transcription in HepG2 human hepatoma cells through an adenosine signal

Dongqin Yang
- Division of Bioinformation, Department of Physiology, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya 663-8501, Japan
Takahiro Yaguchi
- Division of Bioinformation, Department of Physiology, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya 663-8501, Japan
Chun-Ren Lim
- DNA Chip Research Inc., 1-1-43 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan
Youhei Ishizawa
- DNA Chip Research Inc., 1-1-43 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan
Takashi Nakano
- Department of Thoracic Oncology, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya 663-8501, Japan
Tomoyuki Nishizaki
- Division of Bioinformation, Department of Physiology, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya 663-8501, Japan
- Corresponding author. Tel.: +81 798 45 6397; fax: +81 798 45 6649.

Received 14 September 2009; received in revised form 20 October 2009; accepted 20 October 2009. published online 09 November 2009.

Abstract

Extracellular adenosine-induced apoptosis of HepG2 cells, a human hepatoma cell line, in a concentration (0.1–20mM)- and treatment (24–72h)-dependent manner by activating caspase-3, -8, and -9. In the gene expression assay using a DNA microalley, adenosine upregulated mRNAs for tumor necrosis factor (TNF), TNF receptor 1-associated death domain protein (TRADD), TNF related apoptosis-inducing ligand receptor 2 (TRAIL-R2), TRADD/receptor-interacting protein kinase 1 (RIPK1), Fas-associated death domain protein (FADD), and caspase-9, involving activation of caspase-8 and -9 followed by the effector caspase-3. The results of the present study suggest that adenosine induces HepG2 cell apoptosis by activating those caspases as a result from tuning apoptosis-mediator gene transcription.