Cancer Letters
Volume 291, Issue 1 , Pages 76-82, 1 May 2010

Silencing MRP4 by small interfering RNA reverses acquired DDP resistance of gastric cancer cell

  • Yan-Hong Zhang

      Affiliations

    • Department of Oncology, Xuhui Central Hospital, Shanghai 200031, China
    • Corresponding Author InformationCorresponding authors.
    • ,
  • Qing Wu

      Affiliations

    • Department of Oncology, Shanghai First People’s Hospital, Shanghai Jiaotong University, Shanghai 200080, China
    • ,
  • Xiu-Ying Xiao

      Affiliations

    • Department of Oncology, Xuhui Central Hospital, Shanghai 200031, China
    • ,
  • Da-Wei Li

      Affiliations

    • Department of Oncology, Shanghai First People’s Hospital, Shanghai Jiaotong University, Shanghai 200080, China
    • ,
  • Xing-Peng Wang

      Affiliations

    • Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University, Shanghai 200072, China
    • Corresponding Author InformationCorresponding authors.

Received 6 September 2009; received in revised form 1 October 2009; accepted 5 October 2009. published online 02 November 2009.

Abstract 

A cisplatin (DDP) resistant cell line (SGC7901/DDP) from a Chinese gastric cancer cell line (SGC7901) was established by step-increasing DDP treatment, and the resultant cell line showed an over 21.9-fold increased resistance to DDP. To identify the mechanism of DDP resistance, the differential gene expression panel was examined by Affymetrix microarray. Among the identified differential genes, 681 genes expression were increased and 1139 genes were decreased. To confirm these gene changes furtherly, one of the upregulated gene, MRP4 was identified with increased mRNA and protein level of SGC7901/DDP by RT-PCR and Western-blot analysis compared with its parental cell line. By using the small interfering RNA (RNAi) to decrease the MRP4 expression, the DDP resistance phenotype of SGC7901/DDP was reversed. These data suggest that MRP4 is a DDP resistance candidate gene of SGC7901 gastric cancer cell line.

Keywords: Gastric cancer, MRP4, Multi-drug resistance, Chemotherapy, DDP

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PII: S0304-3835(09)00624-7

doi:10.1016/j.canlet.2009.10.003

Cancer Letters
Volume 291, Issue 1 , Pages 76-82, 1 May 2010

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