Upregulated fascin1 in non-small cell lung cancer promotes the migration and invasiveness, but not proliferation

Zhao, Jinbo; Zhou, Yongan; Zhang, Zhipei; Tian, Feng; Ma, Nan; Liu, Tonggang; Gu, Zhongping; Wang, Yunjie

doi:10.1016/j.canlet.2009.09.013

« Previous Next »Cancer Letters
Volume 290, Issue 2 , Pages 238-247, 28 April 2010

Upregulated fascin1 in non-small cell lung cancer promotes the migration and invasiveness, but not proliferation

Jinbo Zhao
- Department of Thoracic Surgery, Tangdu Hospital, Forth Military Medical University, Xi’an 710038, China
- These authors contributed equally to this work.
Yongan Zhou
- Department of Thoracic Surgery, Tangdu Hospital, Forth Military Medical University, Xi’an 710038, China
- These authors contributed equally to this work.
Zhipei Zhang
- Department of Thoracic Surgery, Tangdu Hospital, Forth Military Medical University, Xi’an 710038, China
Feng Tian
- Department of Thoracic Surgery, Tangdu Hospital, Forth Military Medical University, Xi’an 710038, China
Nan Ma
- Department of Ophthalmology, Tangdu Hospital, Forth Military Medical University, Xi’an 710038, China
Tonggang Liu
- Department of Thoracic Surgery, Tangdu Hospital, Forth Military Medical University, Xi’an 710038, China
Zhongping Gu
- Department of Thoracic Surgery, Tangdu Hospital, Forth Military Medical University, Xi’an 710038, China
Yunjie Wang
- Department of Thoracic Surgery, Tangdu Hospital, Forth Military Medical University, Xi’an 710038, China
- Corresponding author. Address: Department of Thoracic Surgery, Tangdu Hospital, 1 Xin Si Road, Xi’an 710038, Shannxi, China. Tel.: +86 29 84777827; fax: +86 29 84777838.

Received 8 July 2009; received in revised form 17 September 2009; accepted 17 September 2009. published online 12 October 2009.

Abstract

Fascin1, an actin-binding protein, is overexpressed in many kinds of tumors. However, its exact role in non-small cell lung cancer (NSCLC) is still unclear. We observed that expression of fascin1 was associated with lymph nodes metastasis and TNM staging but not proliferation in NSCLC by using immunohistochemistry. By generating A549 lung cancer clones stably overexpressing different fascin1 protein, we further confirmed that fascin1 could promote A549 cell migration and invasiveness in vitro and in vivo. Using cell proliferation assay, cell cycle analysis in vitro and tumorigenicity assay in vivo, we showed that fascin1 had little influence on proliferation in A549 cells. These results suggest that fascin1 plays a significant role in NSCLC metastasis and may be a target of intervening to prevent the metastasis of NSCLC.

Keywords: Non-small cell lung cancer, Fascin1, Migration, Invasiveness, Proliferation

Abbreviation: NSCLC, non-small cell lung cancer