Cancer Letters
Volume 286, Issue 2 , Pages 180-188, 28 December 2009

Chemoradiation interactions under reduced oxygen conditions: Cellular characteristics of an in vitro model

  • An Wouters

      Affiliations

    • Laboratory of Cancer Research and Clinical Oncology, Department of Medical Oncology, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium
    • Corresponding Author InformationCorresponding author. Address: Laboratory of Cancer Research and Clinical Oncology, University of Antwerp, Universiteitsplein 1, Building T3.11, 2610 Wilrijk, Belgium. Tel.: +32 (0)3 265 25 76; fax: +32 (0)3 825 05 64.
    • ,
  • Bea Pauwels

      Affiliations

    • Laboratory of Cancer Research and Clinical Oncology, Department of Medical Oncology, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium
    • ,
  • Hilde A.J. Lambrechts

      Affiliations

    • Laboratory of Cancer Research and Clinical Oncology, Department of Medical Oncology, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium
    • ,
  • Greet G.O. Pattyn

      Affiliations

    • Laboratory of Cancer Research and Clinical Oncology, Department of Medical Oncology, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium
    • ,
  • Johan Ides

      Affiliations

    • Laboratory of Cancer Research and Clinical Oncology, Department of Medical Oncology, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium
    • ,
  • Marc Baay

      Affiliations

    • Laboratory of Cancer Research and Clinical Oncology, Department of Medical Oncology, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium
    • ,
  • Paul Meijnders

      Affiliations

    • Department of Radiotherapy, University Radiotherapy Antwerp (URA), Lindendreef 1, 2020 Antwerp, Belgium
    • ,
  • Sylvia Dewilde

      Affiliations

    • Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium
    • ,
  • Jan B. Vermorken

      Affiliations

    • Laboratory of Cancer Research and Clinical Oncology, Department of Medical Oncology, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium
    • ,
  • Filip Lardon

      Affiliations

    • Laboratory of Cancer Research and Clinical Oncology, Department of Medical Oncology, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium

Received 16 February 2009; received in revised form 19 May 2009; accepted 25 May 2009. published online 19 June 2009.

Abstract 

Hypoxic tumour regions often contain viable cells that are more resistant to chemotherapy and/or radiotherapy, making it of key importance to analyse new combination treatments under both normoxic and hypoxic conditions. In this study, the impact of moderate hypoxia and anoxia on cellular characteristics was investigated in isogenic A549 cells differing in p53 status. VEGF expression, doubling time, cell cycle distribution, induction of apoptosis and p53 protein expression were evaluated. Radiation survival curves yielded an oxygen enhancement ratio of 1.16–1.67. In conclusion, an in vitro hypoxia model that will be highly useful to analyse chemoradiation interactions is presented.

Keywords: Hypoxia, p53, Cell cycle, Apoptosis, Radioresistance

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PII: S0304-3835(09)00379-6

doi:10.1016/j.canlet.2009.05.026

Cancer Letters
Volume 286, Issue 2 , Pages 180-188, 28 December 2009

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