Inhibition of CD147 gene expression via RNA interference reduces tumor cell invasion, tumorigenicity and increases chemosensitivity to paclitaxel in HO-8910pm cells

Zou, Wei; Yang, Hong; Hou, Xianghua; Zhang, Wei; Chen, Biliang; Xin, Xiaoyan

doi:10.1016/j.canlet.2006.07.005

« Previous Next »Cancer Letters
Volume 248, Issue 2 , Pages 211-218, 18 April 2007

Inhibition of CD147 gene expression via RNA interference reduces tumor cell invasion, tumorigenicity and increases chemosensitivity to paclitaxel in HO-8910pm cells

Wei Zou
- Department of Obstetrics and Gynecology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, PR China
- W. Zou and H. Yang contributed equally to this work, should be regarded as joint First authors.
Hong Yang
- Department of Obstetrics and Gynecology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, PR China
- W. Zou and H. Yang contributed equally to this work, should be regarded as joint First authors.
Xianghua Hou
- Department of Obstetrics and Gynecology, Xinzhou People’s Hospital, Shanxi 034000, PR China
Wei Zhang
- Department of Obstetrics and Gynecology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, PR China
Biliang Chen
- Department of Obstetrics and Gynecology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, PR China
Xiaoyan Xin
- Department of Obstetrics and Gynecology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, PR China
- Corresponding author.

Received 26 March 2006; received in revised form 31 May 2006; accepted 18 July 2006.

Abstract

Overexpression of extracellular matrix metalloproteinase inducer (EMMPRIN or CD147), a member of the immunoglobulin family and a glycoprotein enriched on the surface of tumor cells, promotes invasion, metastasis, growth and survival of malignant cells, and confers resistance to some chemotherapeutic drugs. Here, we used a human U6 promoter-driven DNA template approach to induce short hairpin RNA (shRNA)-triggered RNA interference (RNAi) to block CD147 gene expression in the human ovarian cancer cell line HO-8910pm. Knockdown of CD147 by shRNA resulted in decrease of the HO-8910pm invasion activity in vitro and tumorigenicity in nude mice. The suppression of CD147 expression also sensitized cells to be more sensitive to paclitaxel. These results suggested that CD147 was an ovarian cancer-related gene and CD147 might be a potential target for therapeutic anti-cancer drugs.

Keywords: CD147, RNAi, Short hairpin RNA, Ovarian cancer