Inhibition of gastric cancer cells associated angiogenesis by 15d-prostaglandin J2 through the downregulation of angiopoietin-1

Fu, Yuan-Gen; Sung, Joseph J.Y.; Wu, Kai-Chun; Bai, Alfa H.C.; Chan, Martin C.W.; Yu, Jun; Fan, Dai-Ming; Leung, Wai K.

doi:10.1016/j.canlet.2005.11.039

« Previous Next »Cancer Letters
Volume 243, Issue 2 , Pages 246-254, 18 November 2006

Inhibition of gastric cancer cells associated angiogenesis by 15d-prostaglandin J₂ through the downregulation of angiopoietin-1

Yuan-Gen Fu
- Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, 30-32 Ngan Shing Street, Shatin, Hong Kong SAR, China
Joseph J.Y. Sung
- Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, 30-32 Ngan Shing Street, Shatin, Hong Kong SAR, China
Kai-Chun Wu
- Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, China
Alfa H.C. Bai
- Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, 30-32 Ngan Shing Street, Shatin, Hong Kong SAR, China
Martin C.W. Chan
- Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, 30-32 Ngan Shing Street, Shatin, Hong Kong SAR, China
Jun Yu
- Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, 30-32 Ngan Shing Street, Shatin, Hong Kong SAR, China
Dai-Ming Fan
- Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, China
Wai K. Leung
- Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, 30-32 Ngan Shing Street, Shatin, Hong Kong SAR, China
- Corresponding author. Tel.: +852 2632 3140; fax: +852 2637 3852.

Received 23 August 2005; received in revised form 22 September 2005; accepted 27 November 2005.

Abstract

Peroxisome proliferator-activated receptor gamma (PPARγ) ligands have been shown to inhibit angiogenesis. We showed that treatment with 15d-PGJ₂, a PPARγ ligand, downregulate the expressions of angiopoietin-1 (Ang-1) in gastric cancer cells MKN45. The medium of MKN45 cells treated with 15d-PGJ₂ significantly inhibited the migration and tube formation of human umbilical vein endothelial cells (HUVECs). Moreover, Matrigel plug assay revealed that 15d-PGJ₂ reduced in vivo angiogenesis induced by MKN45 cells. These modulations were restored by the addition of recombinant Ang-1. Our findings supported that 15d-PGJ₂ suppressed angiogenesis of gastric cancer cells by downregulation of Ang-1.

Keywords: Angiogenesis, Angiopoietin, Gastric cancer, Peroxisome proliferators activated receptor