Methyl protodioscin induces G2/M cell cycle arrest and apoptosis in HepG2 liver cancer cells

Wang, Guanghui; Chen, Haifeng; Huang, Minghui; Wang, Naili; Zhang, Jinchao; Zhang, Yaou; Bai, Ganrong; Fong, Wang-Fun; Yang, Mengsu; Yao, Xinsheng

doi:10.1016/j.canlet.2005.10.050

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Volume 241, Issue 1 , Pages 102-109, 8 September 2006

Methyl protodioscin induces G2/M cell cycle arrest and apoptosis in HepG2 liver cancer cells

Guanghui Wang
- Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China
- Shenzhen Biotech and Health Center, City University of Hong Kong Shenzhen Institute, Shenzhen 518057, People's Republic of China
Haifeng Chen
- Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China
Minghui Huang
- Shenzhen Biotech and Health Center, City University of Hong Kong Shenzhen Institute, Shenzhen 518057, People's Republic of China
- Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, People's Republic of China
Naili Wang
- Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China
- Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, People's Republic of China
- Shenzhen Research Center of Traditional Chinese Medicine and Natural Products, Room 315, Building L, Tsinghua campus, university Town, Shenzhen 518055, People's Republic of China
Jinchao Zhang
- Shenzhen Biotech and Health Center, City University of Hong Kong Shenzhen Institute, Shenzhen 518057, People's Republic of China
- Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, People's Republic of China
Yaou Zhang
- Shenzhen Biotech and Health Center, City University of Hong Kong Shenzhen Institute, Shenzhen 518057, People's Republic of China
- Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, People's Republic of China
Ganrong Bai
- Shenzhen Biotech and Health Center, City University of Hong Kong Shenzhen Institute, Shenzhen 518057, People's Republic of China
- Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, People's Republic of China
Wang-Fun Fong
- Shenzhen Biotech and Health Center, City University of Hong Kong Shenzhen Institute, Shenzhen 518057, People's Republic of China
- Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, People's Republic of China
Mengsu Yang
- Shenzhen Biotech and Health Center, City University of Hong Kong Shenzhen Institute, Shenzhen 518057, People's Republic of China
- Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, People's Republic of China
- Corresponding authors. Address: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, People's Republic of China. Tel.: +852 27887797; fax: +852 27887406.
Xinsheng Yao
- Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China
- Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, People's Republic of China
- Shenzhen Research Center of Traditional Chinese Medicine and Natural Products, Room 315, Building L, Tsinghua campus, university Town, Shenzhen 518055, People's Republic of China
- Address: Shenzhen Research Center of Traditional Chinese Medicine and Natural Products. Room 315, Building L, Tsinghua Campus, University Town, Shenzhen 518055, People's Republic of China. Tel.: +86 755 26036137.

Received 10 April 2005; received in revised form 20 September 2005; accepted 9 October 2005.

Abstract

Methyl protodioscin (NSC-698790) is one of the main bioactive components in the traditional Chinese medicine Dioscorea collettii var. hypoglauca (Dioscoreaceae). In this study, we investigated the anti-proliferative effect of methyl protodioscin on the HepG2 cells and the mechanism of the induced cytotoxicity. Treatment of methyl protodioscin resulted in G2/M arrest and apoptosis in HepG2 cells. These effects were attributed to down-regulation of Cyclin B1 and the signaling pathways leading to up-regulation of Bax and down-regulation of BCL2, suggesting that methyl protodioscin may be a novel anti-mitotic agent.

Keywords: Methyl protodioscin, G₂/M arrest, Apoptosis