Cancer Letters
Volume 229, Issue 1 , Pages 93-99, 8 November 2005

HPV 16 E6 sequence variations in Indian patients with cervical neoplasia

  • Narayanan Sathish

      Affiliations

    • Department of Clinical Virology, Christian Medical College, Vellore 632004, India
    • ,
  • Priya Abraham

      Affiliations

    • Department of Clinical Virology, Christian Medical College, Vellore 632004, India
    • Corresponding Author InformationCorresponding author. Tel.: +91 416 2282772; fax: +91 416 2232035.
    • ,
  • Abraham Peedicayil

      Affiliations

    • Department of Obstetrics and Gynaecology, Christian Medical College, Vellore, India
    • ,
  • Gopalan Sridharan

      Affiliations

    • Department of Clinical Virology, Christian Medical College, Vellore 632004, India
    • ,
  • George Chandy

      Affiliations

    • Department of Clinical Gastroenterology and Hepatology, Christian Medical College, Vellore, India

Received 24 March 2005; received in revised form 15 April 2005; accepted 20 April 2005.

Abstract 

In a cross-sectional study performed between June 2001 and November 2003, the HPV 16 E6 gene of 50 women with cervical neoplasia and 20 cytologically normal women (‘controls’) was sequenced following amplification by PCR. The 350T to G variant was seen in 35 (70%) of 50 patients' isolates while it was seen in only 3 (15%) of the 20 isolates from the ‘controls’. The higher occurrence of the 350G variant among the patients was statistically significant (P<0.01). Isolates from patients were grouped into the European (E), Asian-American (AA) and North-American (NA-1) phylogenetic clusters with 46 (92%) belonging to the E cluster. All the 20 isolates from ‘controls’ belonged to the E cluster. This study suggests an association of the HPV 16 350G variant with a higher risk of cervical neoplasia and a predominance of E lineage strains among Indian HPV 16 isolates.

Keywords: Human papillomavirus (HPV)-16, Cervical neoplasia, E6 variants

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PII: S0304-3835(05)00452-0

doi:10.1016/j.canlet.2005.04.026

Cancer Letters
Volume 229, Issue 1 , Pages 93-99, 8 November 2005

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