Cancer Letters
Volume 234, Issue 2 , Pages 135-142, 28 March 2006

Inhibitory effect of the tea polyphenol, (−)-epigallocatechin gallate, on growth of cervical adenocarcinoma cell lines

Department of Obstetrics and Gynecology, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga 849-8501, Japan

Received 3 February 2005; received in revised form 15 March 2005; accepted 17 March 2005.

Abstract 

To investigate the effect of (−)-epigallocatechin gallate (EGCG) on cervical adenocarcinoma, we performed a cell proliferation assay. TRAP assay is used for telomerase activity, flow cytometry analysis and pKi-67 immunofluoroscein staining in cervical adenocarcinoma cell lines (OMC-4, TMCC-1). Our results showed that EGCG inhibited the proliferation assay, TRAP assay for telomerase activity of both cell lines. Although cell apoptosis was induced, we observed that the expression of pKi-67 was suppressed. Our data suggest that EGCG may be effective for the treatment of cervical adenocarcinoma. The mechanisms of the anti-tumor effects were revealed to be the inhibition of telomerase activity, the induction of apoptosis and cell cycle dysregulation.

Keywords: Cervical adenocarcinoma, EGCG, Telomerase, Apoptosis, pKi-67

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PII: S0304-3835(05)00270-3

doi:10.1016/j.canlet.2005.03.053

Cancer Letters
Volume 234, Issue 2 , Pages 135-142, 28 March 2006

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