Cancer Letters
Volume 229, Issue 1 , Pages 43-48, 8 November 2005

TRAIL and docosahexaenoic acid cooperate to induce HT-29 colon cancer cell death

  • Alena Vaculová

      Affiliations

    • Laboratory of Cytokinetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 612 65 Brno, Czech Republic
    • ,
  • Jiřina Hofmanová

      Affiliations

    • Laboratory of Cytokinetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 612 65 Brno, Czech Republic
    • ,
  • Ladislav Anděra

      Affiliations

    • Laboratory of Cell Signalling and Apoptosis, Institute of Molecular Genetics, Vídeňská 1083, 142 20 Praha 4, Czech Republic
    • ,
  • Alois Kozubík

      Affiliations

    • Laboratory of Cytokinetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 612 65 Brno, Czech Republic
    • Corresponding Author InformationCorresponding author. Tel.: +420 541517182; fax: +420 541211293.

Received 6 November 2004; received in revised form 10 December 2004; accepted 13 December 2004.

Abstract 

The resistance of some cancer cells to TRAIL-induced apoptosis is a major obstacle in successful clinical application of this cytokine. Combination treatment with agents capable of sensitising the cells to TRAIL effects is beneficial for new cancer treatment strategies. Docosahexaenoic acid (DHA) is under intense investigation for its ability to affect cancer cell growth and apoptosis. We demonstrated a modulation of TRAIL-induced apoptosis of HT-29 human colon cancer cells by DHA on the molecular (pro-caspase-3, -8, Bid, PARP cleavage) and cellular (cell viability and adhesion) level. To conclude, TRAIL and DHA were shown to cooperate in the induction of colon cancer cell apoptosis.

Keywords: TRAIL, DHA, Cell death, Colon, Cancer

Abbreviations:: DHA, docosahexaenoic acid, FLIP, FLICE inhibitory protein, MMP, mitochondrial membrane potential, PARP, poly(ADP)ribose polymerase, ROS, reactive oxygen species, TNF, tumour necrosis factor, TRAIL, TNF-related apoptosis-inducing ligand

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PII: S0304-3835(04)00982-6

doi:10.1016/j.canlet.2004.12.016

Cancer Letters
Volume 229, Issue 1 , Pages 43-48, 8 November 2005

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