Cancer Letters
Volume 229, Issue 1 , Pages 1-11, 8 November 2005

Expression of base excision DNA repair genes as a biomarker of oxidative DNA damage

Curriculum in Toxicology and Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, NC 27599, USA

Received 25 November 2004; accepted 1 December 2004.

Abstract 

Oxidative stress induced DNA damage is considered to be the most common insult affecting the genome. Moreover, it is recognized as a common pathway to mutations and is suggested to play a major role in the development of chronic diseases such as cancer. However, current analytical methods used to detect oxidative DNA damage have been hampered by both technical and biological obstacles. These include spurious oxidation during DNA isolation and processing, and the inherent removal of damaged bases by numerous operating DNA repair systems. The removal of oxidized bases is performed predominantly by the base excision repair (BER) pathway and it has been shown that induction of DNA repair genes occurs in response to oxidative stress. Here, we demonstrate the utility of measuring changes in expression of BER genes as a sensitive in vivo biomarker for oxidative DNA damage.

Keywords: DNA damage, Base excision repair (BER), Oxidative stress, Reactive oxygen species (ROS)

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PII: S0304-3835(04)00965-6

doi:10.1016/j.canlet.2004.12.002

Cancer Letters
Volume 229, Issue 1 , Pages 1-11, 8 November 2005

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