Experimental model of pulmonary metastasis treatment with IFNα

Martínez, Cristina; Vicente, Vicente; Josefa Yáñez, Ma; García, Juana Ma; Canteras, Manuel; Alcaraz, Miguel

doi:10.1016/j.canlet.2004.11.047

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Volume 225, Issue 1 , Pages 75-83, 8 July 2005

Experimental model of pulmonary metastasis treatment with IFNα

Cristina Martínez
- Department of Pathology, Faculty of Medicine, University of Murcia, 30100 Espinardo, Murcia, Spain
- Corresponding author. Tel.: +34 96 836 7150; fax: +34 96 836 4150.
Vicente Vicente
- Department of Pathology, Faculty of Medicine, University of Murcia, 30100 Espinardo, Murcia, Spain
M^a Josefa Yáñez
- Department of Pathology, Faculty of Medicine, University of Murcia, 30100 Espinardo, Murcia, Spain
Juana M^a García
- Cell Culture Service, Faculty of Medicine, University of Murcia, 30100 Espinardo, Murcia, Spain
Manuel Canteras
- Department of Biostatistics, Faculty of Medicine, University of Murcia, 30100 Espinardo, Murcia, Spain
Miguel Alcaraz
- Department of Radiology and Physical Medicine, Faculty of Medicine, University of Murcia, 30100 Espinardo, Murcia, Spain

Received 26 July 2004; received in revised form 25 November 2004; accepted 28 November 2004.

Abstract

Melanoma shows the high rates of mortality, preferentially by metastasis, and the failure of chemotherapy. We inoculated 30 B16F10 Swiss mice with 5×10⁵ melanocytes. The mice were then inoculated with 150,000; 300,000; 600,000 and 1,200,000IU/mouse of interferon α. A count of the metastatic nodules of the lung surface and the microscopic study were made by image analysis on five histological sections, calculating the implantation percentage and tumoral growth and invasion index. Treatment with the three highest doses of interferon alpha resulted in a dose-dependent reduction of the number of metastatic nodules and invaded area. A correlation existing between the parameters studied and the different antitumoral effects observed.