T11TS/S-LFA3 induces apoptosis of the brain tumor cells: a new approach to characterise the apoptosis associated genetic changes by arbitrarily primed-PCR

Mukherjee, Joydeep; Ghosh, Anirban; Sarkar, Susobhan; Mazumdar, Malabika; Sarkar, Pallab; Duttagupta, Ashish Kumar; Chaudhuri, Swapna

doi:10.1016/j.canlet.2004.09.014

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Volume 222, Issue 1 , Pages 23-38, 10 May 2005

T11TS/S-LFA3 induces apoptosis of the brain tumor cells: a new approach to characterise the apoptosis associated genetic changes by arbitrarily primed-PCR

Joydeep Mukherjee
- Cellular and Molecular Immunology Lab, Department of Physiology, Dr B. C. Roy Post Graduate Institute of Basic Medical Sciences, Institute of Post Graduate Medical Education and Research (I.P.G.M.&R.), 244 B, A.J.C.Bose Road, Kolkata 700 020, West Bengal, India
Anirban Ghosh
- Cellular and Molecular Immunology Lab, Department of Physiology, Dr B. C. Roy Post Graduate Institute of Basic Medical Sciences, Institute of Post Graduate Medical Education and Research (I.P.G.M.&R.), 244 B, A.J.C.Bose Road, Kolkata 700 020, West Bengal, India
Susobhan Sarkar
- Cellular and Molecular Immunology Lab, Department of Physiology, Dr B. C. Roy Post Graduate Institute of Basic Medical Sciences, Institute of Post Graduate Medical Education and Research (I.P.G.M.&R.), 244 B, A.J.C.Bose Road, Kolkata 700 020, West Bengal, India
Malabika Mazumdar
- Cellular and Molecular Immunology Lab, Department of Physiology, Dr B. C. Roy Post Graduate Institute of Basic Medical Sciences, Institute of Post Graduate Medical Education and Research (I.P.G.M.&R.), 244 B, A.J.C.Bose Road, Kolkata 700 020, West Bengal, India
Pallab Sarkar
- Cellular and Molecular Immunology Lab, Department of Physiology, Dr B. C. Roy Post Graduate Institute of Basic Medical Sciences, Institute of Post Graduate Medical Education and Research (I.P.G.M.&R.), 244 B, A.J.C.Bose Road, Kolkata 700 020, West Bengal, India
Ashish Kumar Duttagupta
- Genetics Research Unit, Department of Zoology, University College of Science, Technology and Agriculture, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700 019, West Bengal, India
Swapna Chaudhuri
- Cellular and Molecular Immunology Lab, Department of Physiology, Dr B. C. Roy Post Graduate Institute of Basic Medical Sciences, Institute of Post Graduate Medical Education and Research (I.P.G.M.&R.), 244 B, A.J.C.Bose Road, Kolkata 700 020, West Bengal, India
- Corresponding author. Tel.: +91 33 2557 3147; fax: +91 33 2223 5181.

Received 10 March 2004; received in revised form 30 August 2004; accepted 3 September 2004.

Abstract

Genetic alterations in ethyl nitrosourea (ENU) induced brain tumor model were analysed by simple PCR based technique with arbitrary primers. T11TS/SLFA3 was established previously as a potent immune stimulator with antineoplastic property in experimental glioma model. The goal of this study was to reveal whether T11TS induces apoptosis of the neural neoplastic cell and to decipher the DNA polymorphism level of the cells undergoing apoptosis. The results clearly establish the apoptogenic role of T11TS/S-LFA3 and along with the detection of cancer associated genomic instability, AP-PCR analysis is useful for the detection of DNA level fragmentation, a unique feature of apoptosis.

Keywords: Brain tumor, Genetic instability, Immunotherapy, T11TS/S-LFA3, Apoptosis, AP-PCR