Cancer Letters
Volume 222, Issue 1 , Pages 113-118, 10 May 2005

Protective effect of local temporary ischemia depends on applied dose of radiation

  • Piotr Walichiewicz

      Affiliations

    • Department of Experimental and Clinical Radiobiology, Institute of Oncology, 44-101 Gliwice, Poland
    • ,
  • Waldemar M. Przybyszewski

      Affiliations

    • Department of Experimental and Clinical Radiobiology, Institute of Oncology, 44-101 Gliwice, Poland
    • Corresponding Author InformationCorresponding author. Tel.: +48 32 2789849; fax: +48 32 2313512.
    • ,
  • Miroslaw Snietura

      Affiliations

    • Department of Experimental and Clinical Radiobiology, Institute of Oncology, 44-101 Gliwice, Poland
    • ,
  • Dariusz Lange

      Affiliations

    • Department of Experimental and Clinical Radiobiology, Institute of Oncology, 44-101 Gliwice, Poland
    • ,
  • Arayik Bkhiyan

      Affiliations

    • Silesian Medical University, Zabrze, Poland
    • ,
  • Maria Widel

      Affiliations

    • Department of Experimental and Clinical Radiobiology, Institute of Oncology, 44-101 Gliwice, Poland

Received 8 June 2004; received in revised form 3 August 2004; accepted 7 September 2004.

Abstract 

The aim of this study was to verify hypothesis that protective effect of local temporary ischemia depends on dose of radiation. 56 male WAG-strain rats were used. Total body irradiation with 3×3 and 3×5Gy was performed. Local temporary ischemia was induced by clamping the tail base. The biochemical parameters were the thiobarbituric acid-reactive substances (TBA-RS). In bone marrow smears the polychromatic erythrocyte (PCE) numbers were counted and the numbers of micronucleated PCEs were analyzed. In small intestines the numbers of crypts were calculated. The levels of TBA-RS in the serum of the animals irradiated with a 3×3Gy dose were significantly different (P<0.002). Also in animals irradiated with a dose of 3×3Gy the numbers of intestinal crypts were different (P<0.05). In animals irradiated with dose 3×5Gy, for analyzed parameters differences did not achieve statistical significance. Local temporary ischaemia provides general protection against radiation damage for lower dose. This protective effect disappeared after applications of a higher dose of radiation.

Keywords: Radiation, Anemia, Ischemic preconditioning, Hypoxia, Lipid peroxidation, TBA-RS

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PII: S0304-3835(04)00711-6

doi:10.1016/j.canlet.2004.09.006

Cancer Letters
Volume 222, Issue 1 , Pages 113-118, 10 May 2005

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