Changes over time in thymidine phosphorylase and dihydropyrimidine dehydrogenase when colon cancer samples are left at room temperature

Ishida, Hideyuki; Tatsuta, Masayuki; Yamamoto, Hirofumi; Furukawa, Hiroshi; Masutani, Seizou; Takemoto, Hiroyoshi; Munakata, Satoru; Miwa, Hideaki; Sekimoto, Mitsugu; Monden, Morito

doi:10.1016/j.canlet.2004.08.036

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Volume 222, Issue 1 , Pages 107-112, 10 May 2005

Changes over time in thymidine phosphorylase and dihydropyrimidine dehydrogenase when colon cancer samples are left at room temperature

Hideyuki Ishida
- Department of Surgery, Osaka Seamen's Insurance Hospital, 1-8-30 Chikukou, Minato-ku, Osaka 552-0021, Japan
- Corresponding author. Tel.: +81 6 6572 5721; fax: +81 6 6573 2531.
Masayuki Tatsuta
- Department of Surgery, Sakai Municipal Hospital, 1-1-1 Minami-Yasui-Cho, Sakai, Osaka 591-0064, Japan
Hirofumi Yamamoto
- Department of Surgery and Clinical Oncology, Graduate School of Medicine, Osaka University, Osaka, Japan
Hiroshi Furukawa
- Department of Surgery, Sakai Municipal Hospital, 1-1-1 Minami-Yasui-Cho, Sakai, Osaka 591-0064, Japan
Seizou Masutani
- Department of Surgery, Sakai Municipal Hospital, 1-1-1 Minami-Yasui-Cho, Sakai, Osaka 591-0064, Japan
Hiroyoshi Takemoto
- Department of Surgery, Sakai Municipal Hospital, 1-1-1 Minami-Yasui-Cho, Sakai, Osaka 591-0064, Japan
Satoru Munakata
- Department of Pathology, Sakai Municipal Hospital, Osaka, Japan
Hideaki Miwa
- Department of Pathology, Izumiotsu Municipal Hospital, Osaka, Japan
Mitsugu Sekimoto
- Department of Surgery and Clinical Oncology, Graduate School of Medicine, Osaka University, Osaka, Japan
Morito Monden
- Department of Surgery and Clinical Oncology, Graduate School of Medicine, Osaka University, Osaka, Japan

Received 29 June 2004; received in revised form 23 August 2004; accepted 31 August 2004.

Abstract

The changes over time in thymidine phosphorylase (TP) and dihydropyrimidine dehydrogenase (DPD) were studied when colon cancer samples were left at room temperature. The TP levels and the TP/DPD ratio showed no changes over time in either the tumor or normal tissues. DPD levels increased over time in the normal tissues (P=0.015), but showed no changes in the tumor tissues. When the DPD level in normal tissues is to be examined, the samples should be frozen as soon as possible. In all other cases, the ELISA results will not be affected if the samples are frozen within 6h after collection.