Transforming growth factor-β1 induces LMO7 while enhancing the invasiveness of rat ascites hepatoma cells

Nakamura, Hiroyuki; Mukai, Mutsuko; Komatsu, Keiko; Tanaka-Okamoto, Miki; Itoh, Yu; Ishizaki, Hiroyoshi; Tatsuta, Masaharu; Inoue, Masahiro; Miyoshi, Jun

doi:10.1016/j.canlet.2004.07.023

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Volume 220, Issue 1 , Pages 95-99, 18 March 2005

Transforming growth factor-β1 induces LMO7 while enhancing the invasiveness of rat ascites hepatoma cells

Hiroyuki Nakamura
- Department of Tumor Biochemistry, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537-8511, Japan
- Corresponding author. Tel.: +81-6972-1181x4402; fax: +81-6-6972-7742.
Mutsuko Mukai
- Department of Tumor Biochemistry, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537-8511, Japan
Keiko Komatsu
- Department of Tumor Biochemistry, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537-8511, Japan
Miki Tanaka-Okamoto
- Department of Molecular Biology, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, >Higashinari-ku, Osaka 537-8511, Japan
Yu Itoh
- KAN Research Institute Inc., 93 chudoji-Awatamachi, Shimogyo-ku, Kyoto 600-8815, Japan
Hiroyoshi Ishizaki
- KAN Research Institute Inc., 93 chudoji-Awatamachi, Shimogyo-ku, Kyoto 600-8815, Japan
Masaharu Tatsuta
- Department of Gastrointestinal Oncology, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537-8511, Japan
Masahiro Inoue
- Department of Tumor Biochemistry, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537-8511, Japan
Jun Miyoshi
- Department of Molecular Biology, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, >Higashinari-ku, Osaka 537-8511, Japan

Received 9 July 2004; accepted 21 July 2004.

Abstract

We have previously shown that transforming growth factor-β1 (TGF-β1) markedly stimulates the invasive capacity of rat ascites hepatoma AH130 W1 cells in vitro and in vivo. A differential hybridization procedure was used to isolate genes that were specifically up-regulated in TGF-β1 treated W1 cells. Among ten independent cDNA clones, we focused on LMO7 and a variant isoform, LMO7S, that was generated by alternative splicing. LMO7 had PDZ and LIM domains, while LMO7S had only PDZ domain. TGF-β1 up-regulated expression levels of LMO7 and LMO7S. LMO7 expression was up-regulated in the highly metastatic clone MM1.

Keywords: TGF-β1, Invasion, Differential hybridization, LMO7

Abbreviations: LMO7, LIM-only protein 7, PCD1, pancreas cancer derived 1, RT-PCR, reverse transcription-PCR