Cancer Letters
Volume 210, Issue 1 , Pages 73-80, 8 July 2004

A new polyclonal antibody that recognizes a human receptor for hyaluronan mediated motility

  • Hiroko Kuwabara

      Affiliations

    • Second Department of Pathology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka 569-8686, Japan
    • Corresponding Author InformationCorresponding author. Tel.: +81-72-683-1221; fax: +81-72-684-6514
    • ,
  • Masahiko Yoneda

      Affiliations

    • Biochemistry and Molecular Biology Laboratory, Aichi Prefectural College of Nursing and Health, Aichi 463-8502, Japan
    • ,
  • Masami Nagai

      Affiliations

    • Department of Internal Medicine, Kagawa University Medical School, Kagawa 761-0793, Japan
    • ,
  • Hana Hayasaki

      Affiliations

    • Department of Anatomy, Osaka Medical College, Osaka 569-8686, Japan
    • ,
  • Hiroshi Mori

      Affiliations

    • Second Department of Pathology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka 569-8686, Japan

Received 24 October 2003; received in revised form 6 January 2004; accepted 9 January 2004.

Abstract 

The receptor for hyaluronan mediated motility (RHAMM), a hyaluronan (HA) binding protein, has been shown to play an important role in the motility and invasiveness of malignant cells. We have developed a polyclonal antibody against human RHAMM. A new polyclonal antibody was raised against a mixture of C-terminal RHAMM, which is capable of binding to HA, and the central domain. The antibody showed immunoreactivity to these two peptides, and detected a 95 kDa protein. Immunohistochemically, RHAMM detected by the antibody was present in the cytoplasm and nucleus of malignant B cells. Binding of HA to RHAMM was almost completely blocked by this antibody. These findings suggest that our antibody recognizes RHAMM protein and is useful for blocking HA binding to RHAMM.

Keywords:  Polyclonal antibody, Receptor for hyaluronan mediated motility, Hyaluronan

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PII: S0304-3835(04)00045-X

doi:10.1016/j.canlet.2004.01.004

Cancer Letters
Volume 210, Issue 1 , Pages 73-80, 8 July 2004

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