Frequent chromosome Y loss in primary, second primary and metastatic squamous cell carcinomas of the head and neck region

Kujawski, Maciej; Jarmuz, Malgorzata; Rydzanicz, Malgorzata; Szukala, Katarzyna; Wierzbicka, Malgorzata; Grenman, Reidar; Golusinski, Wojciech; Szyfter, Krzysztof

doi:10.1016/j.canlet.2003.11.006

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Volume 208, Issue 1 , Pages 95-101, 10 May 2004

Frequent chromosome Y loss in primary, second primary and metastatic squamous cell carcinomas of the head and neck region

Maciej Kujawski
- Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, Poznan 60-479, Poland
- Corresponding author. Tel.: +48-61-8233-011; fax: +48-61-8233-235
Malgorzata Jarmuz
- Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, Poznan 60-479, Poland
Malgorzata Rydzanicz
- Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, Poznan 60-479, Poland
Katarzyna Szukala
- Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, Poznan 60-479, Poland
Malgorzata Wierzbicka
- Otolaryngology Clinic, University of Medical Sciences, Poznan, Poland
Reidar Grenman
- Department of Otorhinolaryngology—Head and Neck Surgery, University of Turku, Turku, Finland
Wojciech Golusinski
- Otolaryngology Clinic, University of Medical Sciences, Poznan, Poland
Krzysztof Szyfter
- Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, Poznan 60-479, Poland
- Otolaryngology Clinic, University of Medical Sciences, Poznan, Poland

Received 5 August 2003; received in revised form 5 November 2003; accepted 8 November 2003.

Abstract

The loss of chromosome Y has often been observed in human solid tumors. This chromosome aberration has been proposed as one of genetic changes predisposing men to squamous cell carcinoma of the head and neck (SCCHN). In this study, using cytogenetic analysis and fluorescence in situ hybridization we analyzed: 16 cell lines derived from primary and recurrent SCCHN, a group of 22 samples derived from of previously analyzed primary larynx tumors and their corresponding metastases and a group of eight multiple primary tumors received from two different locations within the head and neck region of the same patients.

In the majority of analyzed cell lines we found both loss of chromosome Y and SRY-probe signals (68.7% of samples) and these were nearly always found in the analyzed metaphases. The whole chromosome Y was usually lost, but in two cases we observed translocation of this chromosome to chromosomes 1, 3 and 17.

Among all primary tumors, 14 (63.6%) and 15 of their metastases (68.2%) showed a loss of chromosome Y in a prevailing number of analyzed nuclei. Also, in the group of primary tumors and second primary tumors, all samples had a loss of the chromosome Y in the majority of analyzed nuclei.

Keywords: Chromosome Y, Loss, Larynx cancer, Cytogenetics, Fluorescence in situ hybridization, Squamous cell carcinoma