Cancer Letters
Volume 205, Issue 2 , Pages 215-226 , 18 March 2004

Expression and functional characterization of the system l amino acid transporter in KB human oral epidermoid carcinoma cells

  • Jung Hoon Yoon

      Affiliations

    • Oral Biology Research Institute, Chosun University College of Dentistry, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, South Korea
    • ,
  • Youn Bae Kim

      Affiliations

    • Oral Biology Research Institute, Chosun University College of Dentistry, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, South Korea
    • ,
  • Myong Soo Kim

      Affiliations

    • Oral Biology Research Institute, Chosun University College of Dentistry, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, South Korea
    • ,
  • Joo Cheol Park

      Affiliations

    • Oral Biology Research Institute, Chosun University College of Dentistry, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, South Korea
    • ,
  • Joong Ki Kook

      Affiliations

    • Oral Biology Research Institute, Chosun University College of Dentistry, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, South Korea
    • ,
  • Hae Man Jung

      Affiliations

    • Oral Biology Research Institute, Chosun University College of Dentistry, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, South Korea
    • ,
  • Saeng Gon Kim

      Affiliations

    • Oral Biology Research Institute, Chosun University College of Dentistry, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, South Korea
    • ,
  • Hoon Yoo

      Affiliations

    • Oral Biology Research Institute, Chosun University College of Dentistry, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, South Korea
    • ,
  • Yeong Mu Ko

      Affiliations

    • Oral Biology Research Institute, Chosun University College of Dentistry, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, South Korea
    • ,
  • Sang Ho Lee

      Affiliations

    • Oral Biology Research Institute, Chosun University College of Dentistry, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, South Korea
    • ,
  • Bong Young Kim

      Affiliations

    • Department of Pathology, Korea University College of Medicine, Seoul 136-705, South Korea
    • ,
  • Hong Sung Chun

      Affiliations

    • Division of Biological Science, Chosun University, Gwangju 501-759, South Korea
    • ,
  • Yoshikatsu Kanai

      Affiliations

    • Department of Pharmacology and Toxicology, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan
    • ,
  • Hitoshi Endou

      Affiliations

    • Department of Pharmacology and Toxicology, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan
    • ,
  • Do Kyung Kim

      Affiliations

    • Oral Biology Research Institute, Chosun University College of Dentistry, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, South Korea
    • Corresponding Author InformationCorresponding author. Tel.: +82-62-230-6893; fax: +82-62-223-3205

Received 9 March 2003 ,Revised 2 September 2003 ,Accepted 3 October 2003.

References 

  1. Christensen HN. Role of amino acid transport and countertransport in nutrition and metabolism. Physiol. Rev. 1990;70:43–77
  2. McGivan JD, Pastor-Anglada M. Regulatory and molecular aspects of mammalian amino acid transport. Biochem. J. 1994;299:321–334
  3. Oxender DL, Christensen HN. Evidence for two types of mediation of neutral amino acid transport in Ehrlich cells. Nature. 1963;197:765–767
  4. Goldenberg GJ, Lam HY, Begleiter A. Active carrier-mediated transport of melphalan by two separate amino acid transport systems in LPC-1 plasmacytoma cells in vitro. J. Biol. Chem. 1979;254:1057–1064
  5. Lakshmanan M, Goncalves E, Lessly G, Foti D, Robbins J. The transport of thyroxine into mouse neuroblastoma cells, NB41A3: the effect of l-system amino acids. Endocrinology. 1990;126:3245–3250
  6. Blondeau JP, Beslin A, Chantoux F, Francon J. Triiodothyronine is a high-affinity inhibitor of amino acid transport system L1 in cultured astrocytes. J. Neurochem. 1993;60:1407–1413
  7. Su TZ, Lunney E, Campbell G, Oxender DL. Transport of gabapentin, a gamma-amino acid drug, by system l alpha-amino acid transporters: a comparative study in astrocytes, synaptosomes and CHO cells. J. Neurochem. 1995;64:2125–2131
  8. Gomes P, Soares-da-Silva P. l-DOPA transport properties in an immortalized cell line of rat capillary cerebral endothelial cells, RBE 4. Brain Res. 1999;829:143–150
  9. Kanai Y, Endou H. Heterodimeric amino acid transporters: molecular biology and pathological and pharmacological relevance. Curr. Drug Metab. 2001;2:339–354
  10. Kanai Y, Segawa H, Miyamoto K, Uchino H, Takeda E, Endou H. Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98). J. Biol. Chem. 1998;273:23629–23632
  11. Yanagida O, Kanai Y, Chairoungdua A, Kim DK, Segawa H, Nii T, et al.  Human l-type amino acid transporter 1 (LAT1): characterization of function and expression in tumor cell lines. Biochim. Biophys. Acta. 2001;1514:291–302
  12. Uchino H, Kanai Y, Kim DK, Wempe MF, Chairoungdua A, Morimoto E, et al. Transport of amino acid-related compounds mediated by l-type amino acid transporter 1 (LAT1): insights into the mechanisms of substrate recognition. Mol. Pharmacol. 2002;61:729–737
  13. Mannion BA, Kolesnikova TV, Lin S-H, Thompson NL, Hemler ME. The light chain of CD98 is identified as E16/TA1 protein. J. Biol. Chem. 1998;273:33127–33129
  14. Mastroberardino L, Spindler B, Pfeiffer R, Skelly PJ, Loffing J, Shoemaker CB, et al. Amino-acid transport by heterodimers of 4F2hc/CD98 and members of a permease family. Nature. 1998;395:288–291
  15. Pfeiffer R, Spindler B, Loffing J, Skelly PJ, Shoemaker CB, Verrey F. Functional heterodimeric amino acid transporters lacking cysteine residues involved in disulfide bond. Fed. Eur. Biochem. Sci. Lett. 1998;439:157–162
  16. Nakamura E, Sato M, Yang H, Miyagawa F, Harasaki M, Tomita K, et al.  4F2 (CD98) heavy chain is associated covalently with an amino acid transporter and controls intracellular trafficking and membrane topology of 4F2 heterodimer. J. Biol. Chem. 1999;274:3009–3016
  17. Prasad PD, Wang H, Huang H, Kekuda R, Rajan DP, Leibach FH, et al. Human LAT1, a subunit of system L amino acid transporter: molecular cloning and transport function. Biochem. Biophys. Res. Commun. 1999;255:283–288
  18. Sang J, Lim Y-P, Panzia M, Finch P, Thompson NL. TA1, a highly conserved oncofetal complementary DNA from rat hepatoma, encodes an integral membrane protein associated with liver development, carcinogenesis, and cell activation. Cancer Res. 1995;55:1152–1159
  19. Wolf DA, Wang S, Panzia MA, Bassily NH, Thompson NL. Expression of a highly conserved oncofetal gene, TA1/E16, in human colon carcinoma and other primary cancers: homology to Schistosoma mansoni amino acid permease and Caenorhabditis elegans gene products. Cancer Res. 1996;56:5012–5022
  20. Pineda M, Fernandez E, Torrents D, Estevez R, Lopez C, Camps M, et al.  Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an l-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids. J. Biol. Chem. 1999;274:19738–19744
  21. Rossier G, Meier C, Bauch C, Summa V, Sordat B, Verrey F, et al. LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine. J. Biol. Chem. 1999;274:34948–34954
  22. Segawa H, Fukasawa Y, Miyamoto K, Takeda E, Endou H, Kanai Y. Identification and functional characterization of a Na+-independent neutral amino acid transporter with broad substrate selectivity. J. Biol. Chem. 1999;274:19745–19751
  23. Rajan DP, Kekuda R, Huang W, Devoe LD, Leibach FH, Prasad PD, et al. Cloning and functional characterization of a Na+-independent, broad-specific neutral amino acid transporter from mammalian intestine. Biochim. Biophys. Acta. 2000;1463:6–14
  24. Kim DK, Kanai Y, Choi HW, Tangtrongsup S, Chairoungdua A, Babu E, et al.  Characterization of the system L amino acid transporter in T24 human bladder carcinoma cells. Biochim. Biophys. Acta. 2002;1565:112–121
  25. Notani PN. Epidemiology and prevention of head and neck cancer: a global view. In:  Saranath D editors. Contemporary Issues in Oral Cancer. 2000:New Delhi: Oxford University Press; 2000;p. 1–29
  26. Keum YS, Kim J, Lee KH, Park KK, Surh YJ, Lee JM, et al.  Induction of apoptosis and caspase-3 activation by chemopreventive [6]-paradol and structurally related compounds in KB cells. Cancer Lett. 2002;177:41–47
  27. Utsunomiya-Tate N, Endou H, Kanai Y. Cloning and functional characterization of a system ASC-like Na+-dependent neutral amino acid transporter. J. Biol. Chem. 1996;271:14883–14890
  28. Birch DE. Simplified hot start PCR. Nature. 1996;381:445–446
  29. Kim DK, Kanai Y, Matsuo H, Kim JY, Chairoungdua A, Kobayashi Y, et al.  The human T-type amino acid transporter-1: characterization, gene organization, and chromosomal location. Genomics. 2002;79:95–103
  30. Utsunomiya-Tate N, Endou H, Kanai Y. Tissue specific variants of glutamate transporter GLT-1. Fed. Eur. Biochem. Sci. Lett. 1997;416:312–316
  31. Kanai Y, Hediger MA. Primary structure and functional characterization of a high-affinity glutamate transporter. Nature. 1992;360:467–471
  32. Mayanil CSK, George D, Freilich L, Miljan EJ, Mania-Farnell B, McLone DG, et al. Microarray analysis detects novel Pax3 downstream target genes. J. Biol. Chem. 2001;276:49299–49309
  33. Thorens B, Sarkar HK, Kaback HR, Lodish HF. Cloning and functional expression in bacteria of a novel glucose transporter present in liver, intestine, kidney, and beta-pancreatic islet cells. Cell. 1988;55:281–290
  34. Chairoungdua A, Segawa H, Kim JY, Miyamoto K, Haga H, Fukui Y, et al.  Identification of an amino acid transporter associated with the cystinuria-related type II membrane glycoprotein. J. Biol. Chem. 1999;274:28845–28848
  35. Fukasawa Y, Segawa H, Kim JY, Chairoungdua A, Kim DK, Matsuo H, et al.  Identification and characterization of a Na+-independent neutral amino acid transporter that associates with the 4F2 heavy chain and exhibits substrate selectivity for small neutral d- and l-amino acids. J. Biol. Chem. 2000;275:9690–9698
  36. Kim DK, Kanai Y, Chairoungdua A, Matsuo H, Cha SH, Endou H. Expression cloning of a Na+-independent aromatic amino acid transporter with structural similarity to H+/monocarboxylate transporters. J. Biol. Chem. 2001;276:17221–17228
  37. Ohkame H, Masuda H, Ishii Y, Kanai Y. Expression of l-type amino acid transporter 1 (LAT1) and 4F2 heavy chain (4F2hc) in liver tumor lesions of rat models. J. Surg. Oncol. 2001;78:265–271
  38. Matsuo H, Tsukada S, Nakata T, Chairoungdua A, Kim DK, Cha SH, et al.  Expression of a system l neutral amino acid transporter at the blood-brain barrier. NeuroReport. 2000;11:3507–3511
  39. Mizoguchi K, Cha SH, Chairoungdua A, Kim DK, Shigeta Y, Matsuo H, et al.  Human cystinuria-related transporter: localization and functional characterization. Kidney Int. 2001;59:1821–1833
  40. Todd R, Donoff RB, Wong DT. The molecular biology of oral carcinogenesis: toward a tumor progression model. J. Oral. Maxillofac. Surg. 1997;55:613–623
  41. Christensen HN, Handlogten ME, Lam I, Tager HS, Zand R. A bicyclic amino acid to improve discriminations among transport systems. J. Biol. Chem. 1969;244:1510–1520
  42. Sloan JL, Mager S. Cloning and functional expression of a human Na+ and Cl−-dependent neutral and cationic amino acid transporter B0,+. J. Biol. Chem. 1999;274:23740–23745

PII: S0304-3835(03)00687-6

doi: 10.1016/j.canlet.2003.10.009

Cancer Letters
Volume 205, Issue 2 , Pages 215-226 , 18 March 2004

Article Tools

* Image Usage & Resolution