Cancer Letters
Volume 205, Issue 2 , Pages 121-131, 18 March 2004

Two-dimensional electrophoretic protein profile analysis following exposure of human uroepithelial cells to occupational bladder carcinogens

  • Mary Helen Kanitz

      Affiliations

    • Division of Applied Research and Technology, Taft Laboratory, National Institute for Occupational Safety and Health, MS/C-23, 4676 Columbia Parkway, Cincinnati, OH 45226, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1-513-533-8488; fax: +1-513-533-8510
    • ,
  • Santhanam Swaminathan

      Affiliations

    • Department of Pharmacology, University of Wisconsin Medical School, Madison, WI 53706, USA
    • ,
  • Russell E Savage Jr.

      Affiliations

    • Division of Applied Research and Technology, Taft Laboratory, National Institute for Occupational Safety and Health, MS/C-23, 4676 Columbia Parkway, Cincinnati, OH 45226, USA

Received 15 January 2003; received in revised form 29 September 2003; accepted 30 September 2003.

Abstract 

Protein biomarkers to occupational carcinogens were investigated using a transformable human uroepithelial cell system, SV-HUC.PC. SV-HUC.PC was treated with N-hydroxy-4,4′-methylene bis (2-chloroaniline) (N-OH-MOCA) or N-hydroxy-4 aminobiphenyl (N-OH-ABP). Two-dimensional gel electrophoresis of cell lysates compared protein changes across treatments. Increasing N-OH-MOCA resulted in a dose-related increase in protein spots altered. Comparing cell profiles treated with either carcinogen revealed alterations in the expression of nine proteins, identified using the TagIdent database. These demonstrated isoelectric point shift (1) or quantity change (8). Our investigation may be useful in identifying biomarkers of effects of exposure to bladder carcinogens.

Keywords:  Uroepithelial cells, Bladder carcinogen, Two-dimensional gel electrophoresis, Protein biomarkers

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PII: S0304-3835(03)00683-9

doi:10.1016/j.canlet.2003.09.032

Cancer Letters
Volume 205, Issue 2 , Pages 121-131, 18 March 2004

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